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產品名稱：重組豬白細胞介素-8 / CXCL8蛋白
產品型號：rPoIL-8/CXCL8
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組豬白細胞介素-8 / CXCL8蛋白與其它公司提供的重組蛋白不同，rPoIL-8/CXCL8蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組豬白細胞介素-8 / CXCL8蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組豬白細胞介素-8 / CXCL8蛋白

Synonyms (Ser-IL-8)72, GCP/IL-8 Protein I, IL8/NAP1 form III, LYNAP, MDNCF-c, NAF

Species Porcine

Accession P26894

GeneID 396880

Source Escherichia coli.

Molecular Weight 重組豬白細胞介素-8 / CXCL8蛋白Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 78 amino acids.

Quantity 2μg/10μg/1000μg

AA Sequence ARVSAELRCQ CINTHSTPFH PKFIKELRVI ESGPHCENSE IIVKLVNGKE VCLDPKEKWV QKVVQIFLKR TEKQQQQQ

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組豬白細胞介素-8 / CXCL8蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 20-200 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.

Endotoxin 重組豬白細胞介素-8 / CXCL8蛋白Less than 1 EU/μg of rPoIL-8/CXCL8 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組豬白細胞介素-8 / CXCL8蛋白

SDS-PAGE

Reference 1. Modi WS, Dean M, Seuanez HN, et al. 1990. Hum Genet. 84:185-7.
2. Wolff B, Burns AR, Middleton J, et al. 1998. J Exp Med. 188:1757-62.
3. Utgaard JO, Jahnsen FL, Bakka A, et al. 1998. J Exp Med. 188:1751-6.
4. Van Damme J, Rampart M, Conings R, et al. 1990. Eur J Immunol. 20:2113-8.

Background Interleukin-8 (IL-8) is encoded by the IL8 gene and produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. . IL-8 is often associated with inflammation and has been cited as a proinflammatory mediator in gingivitis and psoriasis.

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Synonyms	(Ser-IL-8)72, GCP/IL-8 Protein I, IL8/NAP1 form III, LYNAP, MDNCF-c, NAF
Species	Porcine
Accession	P26894
GeneID	396880
Source	Escherichia coli.
Molecular Weight	重組豬白細胞介素-8 / CXCL8蛋白Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 78 amino acids.
Quantity	2μg/10μg/1000μg
AA Sequence	ARVSAELRCQ CINTHSTPFH PKFIKELRVI ESGPHCENSE IIVKLVNGKE VCLDPKEKWV QKVVQIFLKR TEKQQQQQ
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組豬白細胞介素-8 / CXCL8蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 20-200 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
Endotoxin	重組豬白細胞介素-8 / CXCL8蛋白Less than 1 EU/μg of rPoIL-8/CXCL8 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組豬白細胞介素-8 / CXCL8蛋白
SDS-PAGE
Reference	1. Modi WS, Dean M, Seuanez HN, et al. 1990. Hum Genet. 84:185-7. 2. Wolff B, Burns AR, Middleton J, et al. 1998. J Exp Med. 188:1757-62. 3. Utgaard JO, Jahnsen FL, Bakka A, et al. 1998. J Exp Med. 188:1751-6. 4. Van Damme J, Rampart M, Conings R, et al. 1990. Eur J Immunol. 20:2113-8.
Background	Interleukin-8 (IL-8) is encoded by the IL8 gene and produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. . IL-8 is often associated with inflammation and has been cited as a proinflammatory mediator in gingivitis and psoriasis.

產品名稱：重組豬白細胞介素-8 / CXCL8蛋白