產品詳情
簡單介紹：
重組大鼠腦多巴胺神經營養因子蛋白與其它公司提供的重組蛋白不同，rRtCDNF蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠腦多巴胺神經營養因子蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組大鼠腦多巴胺神經營養因子蛋白
| Species | Rat |
| Accession | P0C5I0 |
| GeneID | 361276 |
| Source | Escherichia coli. |
| Molecular Weight | 重組大鼠腦多巴胺神經營養因子蛋白Approximately 18.8 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids. |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | QGLEAGVRSR ADCEVCKEFL NRFYNSLLTR GIDFSVDTIE EELISFCADT KGKENRLCYY LGATKDSATK ILGEVTRPMS VHMPTVKICE KLKKMDSQIC ELKYEKKLDL ESVDLWKMRV AELKQILHSW GEECRACAEK HDYVNLIKEL APKYVETRPQ TEL |
| Purity | 重組大鼠腦多巴胺神經營養因子蛋白> 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. It is able to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons when immobilized at 5 - 25 μg/mL on a nitrocellulose-coated microplate. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | 重組大鼠腦多巴胺神經營養因子蛋白Less than 0.1 EU/μg of rRtCDNF as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠腦多巴胺神經營養因子蛋白 | |
| SDS-PAGE |
|
